NebNext Adaptors for illumina

[sequencingfan]

Hi all,

anyone know wchich adapters are better for whole genome sequencing, I'm thinking about NebNext loop adapters. Are they better than standard Y shape adapters.?

[nucacidhunter]

I cannot think of any advantage. They add one extra step for protocol and most likely NEB uses them to avoid IP issues.

[sequencingfan]

I read in some article that loop adapters are more effective during ligation and we can expect more mapped reads to reference genome.

[luc]

I could imagine that they are somewhat more efficient (as NEB claims) thanks to the adapters being a single molecule. Perhaps they have advantages for low input applications because of this.
They have the drawback, though, that you can't use them for PCR-free libraries. If you have enough DNA, PCR-free libraries would my suggestion.

[nucacidhunter]

I read in some article that loop adapters are more effective during ligation and we can expect more mapped reads to reference genome

Would you be able to provide link to those articles?