Has anyone had issues with the kapa library quantification kit? I use it often and recently noticed I'm getting high ct values even in my control libraries. Do you leave the kit to thaw to room temperature for 30mins or do you leave it out longer? Recently I prepared a qPCR plate but the qpcr instrument was in use. I placed the plate in the fridge for 1 hr and placed on the qpcr when it freed up. The ct values were higher than expected in my controls but fell within my standard curve. Is this a phenomenon of the kit unhappy at 4degrees? Or is it something else? has anyone else had any issues with the Kapa library quantification kit they can share? I think it's a great kit but not without its issues that most people don't discuss and kapa tech support isn't awfully helpful in this regard either.
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Last edited by seqgirl123; 11-08-2014, 07:48 PM. -
Dear Seqgirl123,
I am a member of the Support & Applications team from Kapa Biosystems and I would be happy to address your concerns.
We recommend thawing our reagents completely while ensuring that they are protected from light. In addition, we generally recommend running the set-up qPCR plate as soon as possible. That being said, if you would be willing to send us your Ct Scores for both your Standards and Samples, we would be happy to analyze your results and discuss via phone or email. if you are interested, please email your results to [email protected].
Best Regards,
Kapa Support
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