Originally posted by robertami
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I agree that the Bioanalyzer Small RNA assay is good for this purpose. It is less than ideal, as miRNAs will run anywhere from 15-40 nt, but it is probably the best thing currently out there. Also, many "small RNA" preps also include tRNA and sometimes the small rRNAs, so as nucacidhunter mentioned an assay such as Picogreen cannot accurately quantify the miRNA in those types of samples.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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