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I was wondering if anyone out there who uses the Agilent Bravo Option-A has or knows where I can get any protocols for it. I am particularly looking for NGS ones. Thanks
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Hi Shakur,
I know it's been a long time since your post. I have been using the Agilent Bravo-Option A in my lab and have been having a mixed experience with it. I'm starting to look into other options, but I'm really curious to hear what your experiences with the Bravo were.
Was the instrument reliable? Did you have many mechanical problems with it? Did you experience any cross contamination?
Anything you could tell me about your experience would be greatly appreciated! -
Hi Santorini,
We haven't had any cross contamination. It has broken down a couple of times but I would have to say the worst part of it has been programming it. I really wish it was a lot easier to code. Some things I wanted to setup I just couldn't do to the amount of time it would take me to write the program since I have other things I need to be doing.
I don't know if this helps any. What options are you looking at by the way?Comment
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Sounds like your experience has been similar to ours. We have been troubleshooting possible contamination issues, but so far it doesn't seem to be a big problem. For us, the main problems have been large delays and downtime for either the instrument breaking down or trying to program.
We are really looking at anything and everything to see what will work well for us. Possible top contenders are: Hamilton STAR, Perkin Sciclone, Versa 1100, BioMek FXp, and Tecan Freedom Evo.
I've been hearing some of these can also be hard to program, but as long as the customer service is more responsive, hopefully it won't be as big of an issue.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...Yesterday, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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