Hello everyone,
I am in the process of setting up a ChIP-seq experiment for my transcription factor of interest. However I still do not fully understand how to preform the percent input method, or fold enrichment methods necessary to validate enrichment of the ChIP'ed sample before library generation. Can anyone recommend or provide a method? We have qPCR primers designed for a region known to be bound by our TF of interest, how do I use these to demonstrate enrichment? Thanks!
I am in the process of setting up a ChIP-seq experiment for my transcription factor of interest. However I still do not fully understand how to preform the percent input method, or fold enrichment methods necessary to validate enrichment of the ChIP'ed sample before library generation. Can anyone recommend or provide a method? We have qPCR primers designed for a region known to be bound by our TF of interest, how do I use these to demonstrate enrichment? Thanks!