Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • Tom_C
    Member
    • Aug 2012
    • 16
    #1

    ChIP-seq percent input qPCR

    Hello everyone,

    I am in the process of setting up a ChIP-seq experiment for my transcription factor of interest. However I still do not fully understand how to preform the percent input method, or fold enrichment methods necessary to validate enrichment of the ChIP'ed sample before library generation. Can anyone recommend or provide a method? We have qPCR primers designed for a region known to be bound by our TF of interest, how do I use these to demonstrate enrichment? Thanks!
    Tags: None
Previous template Next

Latest Articles

Collapse
  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
High-Resolution Sequencing Exposes Hidden Toxoplasma Diversity by SEQadmin2
Started by SEQadmin2, 07-02-2026, 11:08 AM
0 responses
22 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
07-02-2026, 11:08 AM  
New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
23 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
06-30-2026, 05:37 AM  
Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
22 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
06-26-2026, 11:10 AM  
Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
55 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
06-17-2026, 06:09 AM  
View All
Working...