[teuthbrush]

I really don't know why I only can recover that few amount of DNA. So, could you please have any advise or known what could have happened?

J.

Are you positive you're completely resuspending the beads? After my double digests, the BSA in the enzyme made it extremely sticky, and recovery was extremely difficult without extensive vortexing/centrifuging steps. You really need to make sure there's nothing stuck to the sidewalls of your well/tube, and that your bead/DNA mixture is quite homogeneous.

The problems were much less when I changed magnetic plates., and AmpureXP seems to much less sticky than the homemade variety.