Hi
I am planning to amplify short amplicons (~150bp) and then ligating the adapters (so as to skip the enzymatic fragmentation), I am just wondering whether should I do A tailing to the PCR products prior to proceed to adapter ligation or not??
Thanks
I am planning to amplify short amplicons (~150bp) and then ligating the adapters (so as to skip the enzymatic fragmentation), I am just wondering whether should I do A tailing to the PCR products prior to proceed to adapter ligation or not??
Thanks
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