Expected recovery from AxyPrep Mag PCR Clean up

[Squall]

Hi all,

Apologies if this is a silly question, first post.

I was wondering if anyone had an indication of the expected recovery (% of input) expected from the AxyPrep Mag PCR Clean up when used according to manufacture recommendations.

I am hoping to modify the protocol to suit our workflow, and would like to know the "best case scenario" in terms of recovery in the real world.

Many thanks all.

[nucacidhunter]

That depends on size of fragments, lesser for larger fragments. Generally recovery is 80-90 %of input.

[Squall]

Many thanks.

At the moment I am trying to directly compare the manufacturers protocol to a modified protocol for our workflow. We are trying to generate a cut off for small fragment removal at 300.

Thanks again.