Dears all,
We're about to sequence a genome from a small insect. We can get ~500-1000 ng from 5 pooled individuals. But giving wild population should have high, maybe too high variability, we would like to build libraries from single individuals, maybe half of it, to avoid the gut, full of other stuff.
I was reading the Nextera XT DNA library preparation kit. They claim it is able to work with only 1ng of DNA.
Does anybody have some experience with it? Any other advice?
Thanks you a lot
F
We're about to sequence a genome from a small insect. We can get ~500-1000 ng from 5 pooled individuals. But giving wild population should have high, maybe too high variability, we would like to build libraries from single individuals, maybe half of it, to avoid the gut, full of other stuff.
I was reading the Nextera XT DNA library preparation kit. They claim it is able to work with only 1ng of DNA.
Does anybody have some experience with it? Any other advice?
Thanks you a lot
F
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