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  • Gillow
    Junior Member
    • Mar 2010
    • 6

    #1

    Restriction digest of RNA /DNA hybrid

    Has anyone used a restriction enzyme on a 1st strand cDNA synthesis product

    what would I expect if the SSpI RE is used on a viral cDNA 1st strand product if the site is available on a dsDNA sequence of this genome?
  • flxlex
    Moderator
    • Nov 2008
    • 412

    #2
    Perhaps more luck getting an answer in the Sample Prep / Library Generation forum...

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