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Yep, the enzymatic fragmentation products generated by kits from from several vendors do not require any end-repair.
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Hi, I know it is the old post.
I wanted to know have you been sucessful doing end repair and dA tailing in one step?
I tried using a combination of
1. Large Klenow fragment
2. T4 DNA polymerase
3. Klenow exo
4. T PNK
but it didn't work do you think replacing klenow exo with taq polymerase will work?Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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