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  • #16
    Yep, the enzymatic fragmentation products generated by kits from from several vendors do not require any end-repair.

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    • #17
      Kapa hyperplus end repair and A tailing

      Originally posted by aengstrom View Post
      KAPA hyper plus buffer mix contains both dideoxy ATP and other deoxyNTPs (A, T, G, C) along with the enzymes. how do the end repair and A tailing happen in the same step by selecting only the dideoxy A for A tailing(not the dATP (Deoxy ATP))?

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      • #18
        Hi, I know it is the old post.

        I wanted to know have you been sucessful doing end repair and dA tailing in one step?

        I tried using a combination of
        1. Large Klenow fragment
        2. T4 DNA polymerase
        3. Klenow exo
        4. T PNK

        but it didn't work do you think replacing klenow exo with taq polymerase will work?

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