Yep, the enzymatic fragmentation products generated by kits from from several vendors do not require any end-repair.
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Hi, I know it is the old post.
I wanted to know have you been sucessful doing end repair and dA tailing in one step?
I tried using a combination of
1. Large Klenow fragment
2. T4 DNA polymerase
3. Klenow exo
4. T PNK
but it didn't work do you think replacing klenow exo with taq polymerase will work?Comment
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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