low dna concentration of IP samples

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jon123

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Join Date: Jun 2015

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low dna concentration of IP samples

07-08-2015, 08:02 PM

Hi everyone. I am doing preparation of chip-seq sample. After IP and reverse-cross linking, I purified DNA using qiagen columns. But Nanodrop showed 30ng/μl of non-IP control samples, while IP samples showed only 10ng/μl. Furthermore, 260/230 ratio of IP samles was very low, only 0.5.
(260/230 ration of control samples showed normal value, 2.0)
Shoud I proceed to validation using bioanalyzer?

Best regards.

Last edited by jon123; 07-08-2015, 08:05 PM.
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