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  • snotarba
    Junior Member
    • Jul 2015
    • 3
    #1

    RT Enzyme for template switching in SMART cDNA

    Dears,

    does anyone know what is the best commercially available RT (M-MULV) enzyme to add C bases at the end of cDNA for template switching during SMART cDNA sysntesis?

    Thanks a lot!

    Samuele
    Tags: None
    • bagnall.lab
      Junior Member
      • Jun 2015
      • 6
      #2
      check the single cell thread. There has been a lot of discussion recently as new lots SSII seems to be giving some labs a level of bacterial contamination.

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