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  • niceday
    Member
    • Apr 2010
    • 68

    #1

    RNA fragmentation

    Does anyone know of any covaris settings to fragment RNA? I'm trying to avoid chemical fragmentation if possible.
    Thanks.
  • Hamid
    Senior Member
    • Sep 2009
    • 108

    #2
    Hi,

    Please process your RNA samples in 120ul of TE, or buffer EB, using a Covaris MicroTube. Please use the settings of 10%dc/5i/200cpb/75 seconds to generate fragments of around 200 bases.

    Thank you

    hamid

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