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Have anyone her tries the NEB small RNA library prep kit?
How is the result compared to the Illumina TruSeq?
How is the result compared to the Illumina TruSeq?
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There is more info in this thread: http://seqanswers.com/forums/showthread.php?t=60379
Generally both gives similar results down to 2ng miRNA input (fraction of miRNA in total RNA) with a similar workflow but NEB kit costs less. -
Bioo Scientific also offers a similar kit which uses adapters with randomized ends to reduce ligase bias. The issue of ligase bias is pretty well known in the small RNA library prep field, feel free to PM me for more information. I was hesitant to post here because I don't want it to look like a sales pitch, but I truly believe the use of randomized adapters is the way to go with small RNA library prep.
As a disclaimer, I work for Bioo Scientific.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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