We are trying to adapt the ampure xp protocol into an automated library prep system but we're having a few problems over the volumes. We are using NEBNext fragmentase kits to fragment the DNA and the NEBNext DNA sample prep mastermix set 1 to make our libraries, any advice would be gratefully appreciated - Thanks...
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM