Does anyone use Fluidigm C1 instrument on cells using DNASeq script and final library prep for NGS? Our lab has followed their published protocol using a control cancer cell line & we haven't got good alignment of reads across all chromosomes and have run into some other technical issues that makes us think the WGA used on the C1 isn't that good. Part of the problem is that nearly all the published papers use C1 for RNA-Seq while there are very few DNA-Seq papers out there.
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Every WGA method isn't that good. -
Do you use the C1 NextGenSeq? Fluidigm gives the illustra WGA kit as their choice. When using that, it doesn't work to evenly amplify the template. Does anyone use another kit than the one Fluidigm recommends in their protocol or another process that would be better to perform DNA Seq on single cells?Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
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