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What could be the reason for this bioanalyzer results for a total RNA stranded lib prep? I have been using the low mammalian input kit from clontech.
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Could you post a higher-resolution version of this image and an electropherogram of the template RNA itself? This looks like it might be the result of degraded RNA. -
I see the same in my positive control which is suppose to be RNA of good quality.Comment
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They could both be degraded if you had RNase contamination at some step. How do the RNAs look on the Bioanalyzer?Comment
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What organism did the starting RNA material come from? A "spiky" RNA-seq library trace such as this often indicates the presence of high abundance transcripts. It is possible that your ribosomal depletion step was not very efficient.Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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