Hi all,
We just extracted DNA on several samples using Epicenter Masterpure Gram Positive DNA extraction kit. We're going to send it to an NGS service to be library prepped and DNA sequenced, and the NGS service is requesting us perform some quality check on our DNA samples before shipping it to them.
Their criteria are:
DNA sample amount: at least 1.6 micrograms
DNA concentration: at least 20 nanograms/microliter
O.D. 260/280: between 1.8-2.0
It seems that Qubit or Nanodrop allows you to determine DNA concentration and O.D. Are there tools that give you determine the sample amount (in micrograms), or do you just calculate the sample amount from your concentration using simple math?
I'm new to this process - thanks!
- jerrybug109
We just extracted DNA on several samples using Epicenter Masterpure Gram Positive DNA extraction kit. We're going to send it to an NGS service to be library prepped and DNA sequenced, and the NGS service is requesting us perform some quality check on our DNA samples before shipping it to them.
Their criteria are:
DNA sample amount: at least 1.6 micrograms
DNA concentration: at least 20 nanograms/microliter
O.D. 260/280: between 1.8-2.0
It seems that Qubit or Nanodrop allows you to determine DNA concentration and O.D. Are there tools that give you determine the sample amount (in micrograms), or do you just calculate the sample amount from your concentration using simple math?
I'm new to this process - thanks!
- jerrybug109
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