To perform microbiome DNA shotgun sequencing from mouse feces, does one need to worry about non-microbial DNA i.e. mouse/plant? I would use NEB-microbiome enrichment kit if I have too, i.e. if non-microbial DNA reads represent >5% reads. Any comments/help you can provide will be appreciated.
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You can easily filter out mouse in-silico later, without using additional chemistry that might incur bias. But I don't know what proportion of the reads would be non-microbial. -
I don't know about mouse specifically, but other mammals I've seen have host DNA in the 0.1% - 2% range in a fecal sample. The outside is enriched for host material, so if that low level worries you you can scrape a layer off and reduce it. But as Brian says, the reads can be filtered anyway before assembly. Probably preferable compared to feces-scraping!Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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