Hi,
I have used more than recommended total RNA (7 micrograms) for Ribo-zero depletion in plant. Have just finished ds cDNA synthesis. Any suggestions if I do one more Ribo-depletion step with ds cDNA. Has anyone analyzed how much quantity of ribosomal RNA be depleted with 5 ul of rRNA removal mix (Illumina). I am using TruSeq Stranded Total RNA Kit (Illumina).
I have used more than recommended total RNA (7 micrograms) for Ribo-zero depletion in plant. Have just finished ds cDNA synthesis. Any suggestions if I do one more Ribo-depletion step with ds cDNA. Has anyone analyzed how much quantity of ribosomal RNA be depleted with 5 ul of rRNA removal mix (Illumina). I am using TruSeq Stranded Total RNA Kit (Illumina).
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