Hellos group,
I have a technical question, I'm working with a viral (surprise!) genome. It is approx 200kbp in length and is a DNA virus. We will be sequencing this on the Ion PGM and I have a question about library prep.
We will be using the enzymatic shearing protocol which is optimized for e.coli which is 4.6 - 5.6 Mbp so I need to optimize the shear times. So my question is which is the more important factor to start tweaking, the amount of time spent in the reaction or the amount of enzyme used?
Has anyone had any experience with this type of question I would like to know some of the pitfalls before I start burning through sample preps like a mad man.
I have a technical question, I'm working with a viral (surprise!) genome. It is approx 200kbp in length and is a DNA virus. We will be sequencing this on the Ion PGM and I have a question about library prep.
We will be using the enzymatic shearing protocol which is optimized for e.coli which is 4.6 - 5.6 Mbp so I need to optimize the shear times. So my question is which is the more important factor to start tweaking, the amount of time spent in the reaction or the amount of enzyme used?
Has anyone had any experience with this type of question I would like to know some of the pitfalls before I start burning through sample preps like a mad man.
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