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Hi has anyone used EGS or DSG for fixing haematopoietic cells? Trying to optimise fixation of a cell line using EGS first before Formaldehyde due to the structure of the TF complex. Many protocols suggest EGS of 30-45 minutes, i have found even just 20 minutes to be over kill. The formaldehyde is methanol free single ampules. When fixing with just formaldehyde the sonnication profile on the bioanalyser is ideal yet with EGS then formaldehyde i keep seeing 2 peaks, one around 200bp as expected and another around 3kb. Any suggestions?
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM