Hi has anyone used EGS or DSG for fixing haematopoietic cells? Trying to optimise fixation of a cell line using EGS first before Formaldehyde due to the structure of the TF complex. Many protocols suggest EGS of 30-45 minutes, i have found even just 20 minutes to be over kill. The formaldehyde is methanol free single ampules. When fixing with just formaldehyde the sonnication profile on the bioanalyser is ideal yet with EGS then formaldehyde i keep seeing 2 peaks, one around 200bp as expected and another around 3kb. Any suggestions?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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