Hi has anyone used EGS or DSG for fixing haematopoietic cells? Trying to optimise fixation of a cell line using EGS first before Formaldehyde due to the structure of the TF complex. Many protocols suggest EGS of 30-45 minutes, i have found even just 20 minutes to be over kill. The formaldehyde is methanol free single ampules. When fixing with just formaldehyde the sonnication profile on the bioanalyser is ideal yet with EGS then formaldehyde i keep seeing 2 peaks, one around 200bp as expected and another around 3kb. Any suggestions?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM