Hi Everyone,
We recently just processed a few DNA libraries using the Illumina Truseq PCR free kit. When we ran them on Bioanalyzer, one of the libraries came out perfect, with a single peak centered around 500 bp. The others, however, have a secondary shoulder peak ( ~ 375 bp) attached to the main library curve. We have never had this happen to our previous libraries. Has anyone else seen this/know the underlying cause and how to avoid it? Thanks!
We recently just processed a few DNA libraries using the Illumina Truseq PCR free kit. When we ran them on Bioanalyzer, one of the libraries came out perfect, with a single peak centered around 500 bp. The others, however, have a secondary shoulder peak ( ~ 375 bp) attached to the main library curve. We have never had this happen to our previous libraries. Has anyone else seen this/know the underlying cause and how to avoid it? Thanks!
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