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  • sajoshi
    Member
    • Dec 2015
    • 26

    How to interpret this HS DNA library profile

    Please see attached PDF and give your feedback. Are these good quality libraries for RNA seq.

    Thanks,
    Attached Files
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250

    #2
    They do not seem to be good libraries for sequencing. Fragment size distributions are different among libraries indicating issues with library prep which will affect downstream sequencing and analysis negatively. Four of the libraries have high level of prime/adapter-dimers. Libraries are also overloaded for HS Chip.

    Comment

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