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Please see attached PDF and give your feedback. Are these good quality libraries for RNA seq.
Thanks,
Thanks,
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They do not seem to be good libraries for sequencing. Fragment size distributions are different among libraries indicating issues with library prep which will affect downstream sequencing and analysis negatively. Four of the libraries have high level of prime/adapter-dimers. Libraries are also overloaded for HS Chip.
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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