cDNA fragmentation for SOLiD sequencing

[kalu]

Hi everyone

I'll perform a transcriptomic study using the SOLiD system. However, instead of fragmenting total RNA or mRNA, I plan to fragment the cDNA. Briefly, the total RNA was isolated with TriZol + Nucleo Spin II columns, and cDNA synthesis was performed with the SMARTer cDNA synthesis kit (Clontech). We analysed the cDNA with the Agilent and found some fragments in each sample ranging up to 2000bp.

What do you think about fragmenting the cDNA??? I'm afraid I don't have time to perform everything from the beginning...

Thank you very much.
Cheers
Kalu

[snetmcom]

You should treat the sample as a standard DNA library now. If you have access to a covaris, that would probably be your best bet.

[kalu]

Thank you snetmcom! I'll check if will have access to a Covaris.. If I won't, do you have any further suggestion? Thank's again.