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I've heard from some people that SPRI is not a great way to concentrate DNA products. I usually use 0.6x SPRI on 40-50 uL of PCR product and elute into 30-40uL of water. I am wondering if I could concentrate the product further by just using lower volume of water (or TE, or any elution buffer), like maybe 10uL. Anyone here has any experience doing that? A lot of protocols seem to suggest putting the elutted product on a column to concentrate, which I think kinda defeats the purpose of using SPRI...
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I usually take a 50 ul PCR reaction and elute into 22.5 ul. Ten ul seems extreme - you may have problems pipetting up the liquid without beads. -
I elute 50 uL PCR products in 20 uL pretty regularly without any issues or loss in yield. When going below 20 uL, I start to see a drop in total recovery.Josh KinmanComment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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