Bioanalyzer smallRNA results

[sajoshi]

Please see attached bioanalyzer profile for a smallRNA chip. I ran the same samples twice, but the ladder is giving some problem. I prepared the gel two times separately thinking that the gel might have some problem. Can you provide your feedback as to why I am getting these results.

I was planning to use these samples for Clontech smart ultra low input kit for RNA.

Thanks

[nucacidhunter]

Interpreting results without a good ladder run is difficult. Some reasons could be following:
1- Faulty reagents
2- Dirty pin
3- Other instrument issues

Sample related issues:
1- Presence of contaminants such as proteins and salts
2- Residual gDNA
3- Degraded sample

I wonder why you run samples on Small RNA Chip. If you are going to prepare RNA-Seq library, they should be run on RNA Pico or Nano Chip depending on concentrations. You would need RIN from those Chips to decide on fragmentation time. Contacting tech support should resolve instrument or reagent related issues.