Mulitplexing

[RezaFalsafi]

Hello everyone,
Can I use the Nextera XT kit 96 index with TRuseq RNA ?
Basically my question is: can i use these Nextera XT dNA indices, with rNA as starting material? these are the only index kit sold separately by Illumina
As why I want to do so, a long explanation, and by the way I usually prep 70-80 samples
Or if anyone knows of any good 96 unique indices preferably 6 nt please let me know, I do know of Bioo by the way. And again for RNAseq
Thanks a lot in advance

[nucacidhunter]

You need adapters for ligation to ds-cDNA. Nextera index kit contains only oligos. Other option is NEBNext Multiplex Oligos for Illumina. NEB supplies 24 single index and for higher indexing one have to use dual indexing primers.

[RezaFalsafi]

Thank you very much for the reply. I did not know Nextera contains only oilgos
NEB wont work for me as I need Y shaped adapters that can be ligated as in the Truseq protocol, or others. NEB ones are PCR generated
Thanks