After obtaining the amplified adapter-ligated cDNA fragments, the Illumina protocol suggests the cloning of 4% of the volume of the library into a sequencing vector so in order to sequence the individual colonies. I have size selected 150-350 bps cDNA fragments. What will conventional sequencing tell me? The protocol says u can verify that insert sequences are from the genomic source DNA. But I don't get how. Can anyone please explain it to me?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
