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After obtaining the amplified adapter-ligated cDNA fragments, the Illumina protocol suggests the cloning of 4% of the volume of the library into a sequencing vector so in order to sequence the individual colonies. I have size selected 150-350 bps cDNA fragments. What will conventional sequencing tell me? The protocol says u can verify that insert sequences are from the genomic source DNA. But I don't get how. Can anyone please explain it to me?
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM