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Hi all,
When I used Tapestation last time, I accidentally forgot to remove the caps of the tubes. There was error warning and after I cleaned up the stuck tips I re-ran some RNAs (only 4 out of 16 lanes in the tape can be detected). The run seemed work normally and I did get my results. The only thing I am worried is that apart from empty control and ladder, my two RNA samples (Arabidopsis roots) got RIN 10.0. While the RNA gel looked great, I still can't believe they got 10! The ladder looks fine and my RNA concentration is around 100ng/ul, lower than what I got from Qubit but comparable.
So I want to ask do you think my mistake (forgot to remove cap) can affect the performance of the machine? Did anyone have the same experience?
When I used Tapestation last time, I accidentally forgot to remove the caps of the tubes. There was error warning and after I cleaned up the stuck tips I re-ran some RNAs (only 4 out of 16 lanes in the tape can be detected). The run seemed work normally and I did get my results. The only thing I am worried is that apart from empty control and ladder, my two RNA samples (Arabidopsis roots) got RIN 10.0. While the RNA gel looked great, I still can't believe they got 10! The ladder looks fine and my RNA concentration is around 100ng/ul, lower than what I got from Qubit but comparable.
So I want to ask do you think my mistake (forgot to remove cap) can affect the performance of the machine? Did anyone have the same experience?
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