I'm looking into using the xGen lockdown kit to select for a panel of specific genes from our RNA seq samples. These are low input samples, so my normal workflow is doing about 13 cycles of preamp, then pool and SPRI clean. Then I take that into Nextera, which is then amplified again with the Nextera handles for 12 cycles. This usually produced me with sufficient DNA for a NextSeq run (1-4nM, maybe about 10-20uL total).
I'm looking into doing a gene enrichment with the xGEN kit after the preamp. but the recommended input of the kit seems really high. It asks for 500ng of material, while my preamp material is usually around 1-2ng/uL (15uL total). Does anybody have any idea whether the kit would still work with much lower input? Or is there a different kit that would be more suited for this purpose? (the libraries are already barcoded. so no additional barcoding is necessary.)
I'm looking into doing a gene enrichment with the xGEN kit after the preamp. but the recommended input of the kit seems really high. It asks for 500ng of material, while my preamp material is usually around 1-2ng/uL (15uL total). Does anybody have any idea whether the kit would still work with much lower input? Or is there a different kit that would be more suited for this purpose? (the libraries are already barcoded. so no additional barcoding is necessary.)
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