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Closer examination of optical and current signals indicates:
1- Current drops at the start of elution which is normal
2- Elution times are 7:31 and 52:56 for lanes 2 and 3 , respectively, this also is consistent for selected size ranges
3- There is no optical signal after start of elution, abnormal
Suggested actions:
1- Since you do not have access to a BA, if fragments have adapters you may check size range by running PCR using a fraction of eluates and visualising amplicon in gel.
2- Reviewing if the correct loading buffer was used for the cassette type
3- Contacting Sage Science tech support (there could be a hardware malfunction)
1- Current drops at the start of elution which is normal
2- Elution times are 7:31 and 52:56 for lanes 2 and 3 , respectively, this also is consistent for selected size ranges
3- There is no optical signal after start of elution, abnormal
Suggested actions:
1- Since you do not have access to a BA, if fragments have adapters you may check size range by running PCR using a fraction of eluates and visualising amplicon in gel.
2- Reviewing if the correct loading buffer was used for the cassette type
3- Contacting Sage Science tech support (there could be a hardware malfunction)
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