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Anyone got any useful advice for what to do with extra DNA 1000 chips for the Bioanalyzer? We've got at least 3 dozen extra collecting in our drawers. What's everyone do with their extras?
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If you are using the BA for High Sensitivity assays still, you can buy High Sensitivity reagents and use them with the DNA 1000 chips. You just need to load the chip according to the High Sensitivity protocol and disregard the well labels on the DNA 1000 chip (ladder will go in the well that is labeled sample 12 and gel dye matrix in the well for the ladder).Josh Kinman -
To expand on this a little, it appears that all Bioanalyzer DNA and RNA chips are the same chip, just with a different sticker.Originally posted by jdk787 View PostComment
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That's super useful! Thanks, I'll be sure to start doing that.Comment
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Thank you jdk787 & kerplunk412! Very helpful!SeqDavisComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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