Tweet
Hi all. On a different note...I do not really understand how one is supposed to combine the N7 and S5 primers in the QIAseq Targeted DNA Panel. Do they have to be combined in a TruSeq-like fashion? So different N7s dispensed by rows and different S5s dispensed by columns?
-
Last edited by thiNGS; 04-05-2019, 06:54 AM. -
Hi thiNGS,
To combine N7## adapter and S5## index primer in 96-well format, yes different N7## dispensed by rows and different S5## dispensed by columns.Comment
-
Hi everyone,
our genetic testing software 'Seamless NGS' offers an easy-to-use workflow for the analysis of Qiagen's QiaSeq panels.
Before mapping the reads, the common sequence and the UMIs are clipped away. After mapping, the UMIs sequences are properly used to correct for PCR clones and the single amplicon primer is clipped away to ensure correct and unbiased variant calling. The workflow was developed, tested and validated together with a trustworthy pathological laboratory at one of the oldest university hospitals in Germany. Having long-lasting experience in that field, together with them, ecSeq was able to get the most out of the QiaSeq panel.
If you are interested in our solution, please check www.seamless-ngs.com and/or write us a short email ([email protected]) to make an appointment for a personal live demo (online).
Last edited by ecSeq Bioinformatics; 05-13-2019, 01:16 AM.ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).Comment
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment