Hi alla!
Anyone has been working with QIAseq targeted DNA Panel??
It is our first trial and I'm not very glad with the libraries profiles on Bioanalyzer... We have performed a first experiment with two fresh frozen samples (tubes 1 and 2, input 50 ng DNA) and their FFPE partners (at 2 different Input amount; 100 ng and 250 ng, tubes 3, 4, 5 i 6).
What do you think of the library profile? It seems that there are some large fragments in our libraries...any idea?For the fresh frozen it could be an issue related to the PCR...but in the FFPE?? (It doesn't seem to be beads, in my opinion).
Here are the profiles. Thanks a lot in advance!
Anyone has been working with QIAseq targeted DNA Panel??
It is our first trial and I'm not very glad with the libraries profiles on Bioanalyzer... We have performed a first experiment with two fresh frozen samples (tubes 1 and 2, input 50 ng DNA) and their FFPE partners (at 2 different Input amount; 100 ng and 250 ng, tubes 3, 4, 5 i 6).
What do you think of the library profile? It seems that there are some large fragments in our libraries...any idea?For the fresh frozen it could be an issue related to the PCR...but in the FFPE?? (It doesn't seem to be beads, in my opinion).
Here are the profiles. Thanks a lot in advance!
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