Hello everyone,
I'm actually a newbie in this field, and this forum has helped me a lot with some issues.
I've been reading a few Illumina protocols, like TruSeq nano or TruSeq DNA PCR-Free, and in both protocolos there is a dual size selection.
I want to know why there is a bead dilution step before the large fragment removal?. For example,
109.25 ul beads + 74.75 PCR grade water = 184 ul final volume
Then you must use 160 ul of this mix with your sample (equivalent to 95 ul beads)
Is not simplier to add this equivalent bead volume to your sample, regarding to get the same finale volume? (in this case 260 ul)
I hope I've been clear enough.
Thanks!
I'm actually a newbie in this field, and this forum has helped me a lot with some issues.
I've been reading a few Illumina protocols, like TruSeq nano or TruSeq DNA PCR-Free, and in both protocolos there is a dual size selection.
I want to know why there is a bead dilution step before the large fragment removal?. For example,
109.25 ul beads + 74.75 PCR grade water = 184 ul final volume
Then you must use 160 ul of this mix with your sample (equivalent to 95 ul beads)
Is not simplier to add this equivalent bead volume to your sample, regarding to get the same finale volume? (in this case 260 ul)
I hope I've been clear enough.
Thanks!
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