An mRNA profile on Bioanalyzer can take slightly unusual forms. Rarely do we see a perfect clean hump (see attached). Sometimes we get a hump with many jagged sharp peaks and within the same sample set the jagged sharp peaks disappear or are lessened. See the samples attached where the top three samples have a more smooth hump compared to the others. What are these jagged sharp peaks? Are certain mRNAs selected for? (We used Ribo-Zero for rRNA depletion from the Total RNA)
These samples were submitted to the core so I don't know the underlying experiment which may be affecting the mRNA profile.
The researcher did notice a difference in sequence data coming from these three samples. They said "There seems to be a large proportion (30%) of unmappable (unidentifiable) reads in 3 particular libraries out of the 9 Borrelia libraries. I am wondering if there may be anything out of the ordinary for these 3 libraries during library construction and/or sequencing (e.g. RNA quantity, etc.)". Nothing was strange about the library prep protocol; the only thing we noticed was these mRNA profiles.
These samples were submitted to the core so I don't know the underlying experiment which may be affecting the mRNA profile.
The researcher did notice a difference in sequence data coming from these three samples. They said "There seems to be a large proportion (30%) of unmappable (unidentifiable) reads in 3 particular libraries out of the 9 Borrelia libraries. I am wondering if there may be anything out of the ordinary for these 3 libraries during library construction and/or sequencing (e.g. RNA quantity, etc.)". Nothing was strange about the library prep protocol; the only thing we noticed was these mRNA profiles.
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