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  • SeqTroubles
    Member
    • Sep 2016
    • 20

    HS Bioanalyzer peak drops into negative

    Hi All,

    I am after performing a nextera xt library prep and I am running a HS chip at the moment. The samples produce weak scratchy peaks but when they come back down it goes into the negative space.

    Now the kit I am using is expired but I made fresh gel today and the ladder had worked perfect. Is it worth re-testing with a fresh kit?

    Thanks
    Last edited by SeqTroubles; 03-08-2017, 06:27 AM.

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