I'm having a lot of issues with NuGEN's Ovation 3'DGE and RNA-Seq library preparation kits. No matter what I do, I can't seem to generate any libraries for sequencing. Has anyone had a similar problem? When I use the mRNA Seq prep kit from Illumina and try it on the same samples I'm getting great libraries....hmmm.....frustrated....
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are you using Illumina kit to generate library itself, or your own reagents? Anyway, there is a report in Biotechniques that shows that pretreatment of ovation-generated ds cDNA with S1 nuclease increases efficiency of library generation. Their claim is that it's due to hairpins at the ends of DNA fragments that obviously would affect any end repair /adaptor ligation. S1 ligase will get rid of the ssDNA, so should solve the problem.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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