Hi, saw some library kits using sequential adatation process, first to add adapter to the 5 ' prime and then 3'prime, just wondering how it works, many thanks in advance!
-
-
Hello,
This kind of protocol is typical of smallRNAseq.
It uses a truncated T4 RNA ligase 2 and a modified 3' adapter.
Trunc. T4 RNA ligase is unable to adenylate the 5' end of its substrate so it uses the modification of the 3' primer (rApp) to perform this step.
The second ligation uses a classical RNA ligase.
I wasn't aware of such protocols used for RNAseq... -
Thanks!
Swift Bio for example using this strategy to build their Libraries, for DNAseq and RNAseq. It should be different for DNA and RNA application, right?Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
Comment