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This thread now more appropriately reposted, by the author, in the Illumina/ Solexa forum. -
Clonal Amplification/ Kinetic Exclusion
Newer generation Illumina platforms with patterened flowcells use kinetic exclusion to achieve a high degree of clonal chastity. From what I understand, the amplification method is still bridge, but is driven by isothermal RPA, so there is no need for the chemical denaturation and renaturation steps of old.
My question is, can anyone comment on the final number of copies of the clonally amplified material within any given cluster? An although it possibly differs from instrument to instrument, what is the feature size of any given patterned location: what the density of the amplified molecule would therefore be?
Does the signal generated go through a 'photomultiplier' prior to analysis, which I imagine would reduce the need for a strong raw signal, and so would better accommodate a lower level of amplification/ template density during the clonal amplification?
Thanks for any insight.
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