high throughput DNA size selection?
Hi! I'm asking is there any method out for a large quantity of DNA size selection, something like with 96-well plate. The traditional way is to excise agaroase gel and do DNA purification, but it's too labor-intensive for hundreds of samples. I think it would be perfect to do size selection in a 96-well plate. But something like Sephadex seems not able to do such selection (e.g. 250-300bp). Any idea? Thanks!
-
-
Ampure has a protocol for 96 and 384 well plates. Link to protocol (PDF):
-
For tighter sizes pippin or Caliper labchip XT although no plate format for those..Comment
-
thanks. but that's not size selection like cutting a narrow band from the gel, but rather just DNA purification.Originally posted by Zaag View PostComment
-
You can use the ratio of AMPure beads to sample to select a specific size range without using a gel. Since the beads are in a PEG/salt solution, altering the ratio of beads/sample will solubilize different sizes of DNA fragments. For example, using 0.6X AMPure beads removes DNA <500bp and using 1.2X removes DNA <150bp. Using two different ratios of beads will allow you to select a certain size range of your library. Using a 0.6X ratio will cause binding of DNA fragments >500bp, but the smaller fragments will still remain in the supernatant. You can take that supernatant and add it to a higher concentration of AMPure beads and then take the DNA bound to the beads to get a size selection based on the ratios you used. With the 0.6X and 1.2X ratios, your library would range from 150-500bp. Altering the ratios by 0.1X steps will allow you to collect narrower size ranges. This type of size selection isn't extremely accurate so you should only use it if appropriate for your application. Be sure to test this out on some 100bp ladder or something similar since there is a little bit of lot-to-lot variation.Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...Yesterday, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM -
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM
Comment