Sera-Mag oligo-dT beads

[martona]

Hi everybody,

We're starting the cDNA library preparation for 454 but first we need to isolate the mRNA from the total RNA.
We bought the Sera-Mag oligo-dT beads but we have no experience in using them...anyone of you knows how to use them? the right buffers, time of hybridization?
We used the Dynabeads before...can we use the same buffers and protocol?

We sent an email to the sales tech but we don't get any answer yet.

Thank you!

[treebeard]

martona,

The protocol we use for our RNA-seq (Illumina-based) work is at the link below. The libraries have very low rRNA representation, and we have seen excellent reproducibility among technical replicates.

http://openwetware.org/wiki/Random_Lab_Methods

[martona]

Thank you treebeard!!!! Really useful!