Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • JWL
    Junior Member
    • Jan 2012
    • 1

    QIAseq FX Enzyme Mix (for human gDNA)

    Exploring the possibility of using this kit for our hyb-capture library prep front-end process. Does anyone know the specific enzymes that make up the cocktails? As well, is the enzymatic fragmentation random or does it target specific sequences? Is it known what the fragmentation enzymes are? Thanks
  • thermophile
    Senior Member
    • Apr 2015
    • 243

    #2
    My Qiagen rep was just here talking about this. He claimed that it isn't sequence specific cleaving at all but beyond that I couldn't get much info out of him. Showed some data comparing sequencing sites to covaris sheared DNA. They didn't have any company data on what I'd call high gc genomes so he's going send me a kit to try on some 75% GC streptococcus
    Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.

    Comment

    Latest Articles

    Collapse

    • mylaser
      Reply to Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by mylaser
      Kheloyar – Everything You Need to Know About Kheloyaar Login and Kheoyar Id
      If you are looking for an online gaming platform that offers a user-friendly experience, Kheloyar has become a name that many users search for. Whether you're interested in creating a new account, accessing your dashboard through Kheloyaar Login, or learning how to obtain a Kheoyar Id, understanding the platform's features and account process is essential.
      This guide explains everything you need to know about...
      Yesterday, 01:13 AM
    • SEQadmin2
      Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by SEQadmin2



      Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
      ...
      07-09-2026, 11:10 AM
    • SEQadmin2
      Cancer Drug Resistance: The Lingering Barrier to Rising Survival
      by SEQadmin2



      Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

      There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
      07-08-2026, 05:17 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 07-09-2026, 10:04 AM
    0 responses
    19 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-08-2026, 10:08 AM
    0 responses
    11 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-07-2026, 11:05 AM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-02-2026, 11:08 AM
    0 responses
    31 views
    0 reactions
    Last Post SEQadmin2  
    Working...