Hi,
I was wondering whether anybody has ever wanted to sequence tRNAs? I would be grateful for any information.
I am under the impression cDNA synthesis would be impaired by the modifications and secondary structures of tRNAs, so any tips of how to avoid those pitfalls are greatly appreciated.
I am quite new to this, and I go in right at the deep end!
Thanks
Maike
I was wondering whether anybody has ever wanted to sequence tRNAs? I would be grateful for any information.
I am under the impression cDNA synthesis would be impaired by the modifications and secondary structures of tRNAs, so any tips of how to avoid those pitfalls are greatly appreciated.
I am quite new to this, and I go in right at the deep end!
Thanks
Maike
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