Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Immunity
    Junior Member
    • Nov 2017
    • 2

    #1

    Targeted RNASeq procotols for viral sequencing

    Hello,

    I am starting a project on which I would like to detect viral RNA (herpes) in human tissue. Essentially I would extract the RNA, perform targeted amplification and library prep for multiple viruses and then sequence. I think I have an idea of the bioinformatic system to use.
    However, I am not really sure where to start on the library prep issue (i.e. which library prep system to use). I've looked into ScripSeq which sounded promising but seems very expensive.

    Is there a "custom" protocol/template out there than I can use to accomplish these goals. My main concerns are (if i would to start with known herpes primers, how would I successfully make a library that contain adapters and index for multiplex?)

    Thank you and excuse my naive questions. I am not new to using NGS for already commercially prepared custom gene panels (i.e. Qiagen targeted RNASeq panels) but I am new in developing custom assays so your help would be greatly appreciated.
  • Immunity
    Junior Member
    • Nov 2017
    • 2

    #2
    I found out what I needed if anyone is interested. It is called (16S Metagenomic sequencing library preparation). It is made for 16S rRNA targeted amplification, however the protocol can easily be adjusted for any targeted region on any genome. all you need is to attach illumina adapter overhang sequences to the targeting Forward and Reverse primers, amplify, clean up, amplify again with indexes, clean up and sequence.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Working...