Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Immunity
    Junior Member
    • Nov 2017
    • 2

    Targeted RNASeq procotols for viral sequencing

    Hello,

    I am starting a project on which I would like to detect viral RNA (herpes) in human tissue. Essentially I would extract the RNA, perform targeted amplification and library prep for multiple viruses and then sequence. I think I have an idea of the bioinformatic system to use.
    However, I am not really sure where to start on the library prep issue (i.e. which library prep system to use). I've looked into ScripSeq which sounded promising but seems very expensive.

    Is there a "custom" protocol/template out there than I can use to accomplish these goals. My main concerns are (if i would to start with known herpes primers, how would I successfully make a library that contain adapters and index for multiplex?)

    Thank you and excuse my naive questions. I am not new to using NGS for already commercially prepared custom gene panels (i.e. Qiagen targeted RNASeq panels) but I am new in developing custom assays so your help would be greatly appreciated.
  • Immunity
    Junior Member
    • Nov 2017
    • 2

    #2
    I found out what I needed if anyone is interested. It is called (16S Metagenomic sequencing library preparation). It is made for 16S rRNA targeted amplification, however the protocol can easily be adjusted for any targeted region on any genome. all you need is to attach illumina adapter overhang sequences to the targeting Forward and Reverse primers, amplify, clean up, amplify again with indexes, clean up and sequence.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


      Here are nine questions we think about, in roughly the order they matter, before...
      Yesterday, 07:11 AM
    • SEQadmin2
      From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
      by SEQadmin2


      Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


      The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
      ...
      06-02-2026, 10:05 AM
    • SEQadmin2
      Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends
      by SEQadmin2


      With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.


      Introduction

      Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
      05-22-2026, 06:42 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    16 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-09-2026, 11:58 AM
    0 responses
    37 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-05-2026, 10:09 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-04-2026, 08:59 AM
    0 responses
    49 views
    0 reactions
    Last Post SEQadmin2  
    Working...